Process for PRF™ Duo Quattro™ Dental PRF System

Process for PRF™ Duo Quattro™ Dental PRF System

The Process for PRF™ Duo Quattro™ Dental PRF System was developed to give clinicians a complete, ready-to-use PRF solution explicitly tailored for dental bone grafting and periodontal surgery. Designed by the inventor of PRF - Dr. Joseph Choukroun, this system brings together refined instruments, clinically validated tubes, and streamlined workflows shaped by more than twenty years of clinical research.

Two kit configurations are available to meet the demands of different practices.

• The Starter Kit includes all materials needed to create A-PRF™ membranes, plugs, ponchos, and S-PRF™ plasma for sticky bone.
• The Professional Kit includes everything in the Starter Kit plus an additional set of instrumentation to support consecutive surgeries without procedural downtime.

Every component from the tubes to the instruments has been evaluated by a network of dozens of clinicians and educators, resulting in the most refined and effective PRF system on the market. The clinical studies conducted on the Duo Quattro™ Dental PRF System highlight the reliability and performance of the official A-PRF™ and S-PRF™ tubes, which were benchmarked in an independent Tube Comparison Study. The system’s integrated design ensures that clinicians can achieve consistent, high-quality PRF results with remarkable predictability by using the Low-Speed Centrifugation Concept (LSCC), developed by Dr. Choukroun and his colleagues.

Dr. Choukroun’s protocols have been fine-tuned over decades, and the components included in the kit work in harmony, providing the highest-quality PRF possible. The Process for PRF™ Duo Quattro™ Dental PRF System offers a dependable, clinically validated solution for producing high-potency clots and plasma formulations. It remains the most trusted and widely adopted PRF system used in modern dental surgery.

Duo Quattro™ Advanced PRF Centrifuge

At the core of the Process for PRF™ ecosystem is the Duo Quattro™ Advanced PRF Centrifuge, the only centrifuge engineered and approved by Dr. Joseph Choukroun for PRF production. Unlike generic centrifuges repurposed for dental use, the Duo Quattro™ was built specifically for PRF, incorporating the mechanical precision and stability required for dependable clot and plasma formation.

The unit is uniquely authorized to spin A-PRF™ red tubes and S-PRF™ green tubes, making it the central piece of equipment for practices committed to using official PRF protocols for dentistry. Its brushless motor, self-calibrating rotor, and reinforced construction provide long-term accuracy and smooth operation, while pre-programmed PRF settings ensure effortless workflow. Fully adjustable programming also allows clinicians to adapt the centrifuge as new research emerges.

Decades of independent clinical studies support the Duo Quattro’s™ performance, demonstrating its ability to consistently yield high-quality PRF suitable for a wide range of regenerative procedures. The centrifuge is backed by a 2-year warranty and a satisfaction guarantee, emphasizing its durability and professional-grade reliability.

Simple to use, easy to maintain, and engineered specifically for PRF biology, the Duo Quattro™ remains the centrifuge of choice among leading educators, advanced users, and key opinion leaders worldwide.

Horizontal Centrifugation Misconceptions

The growing preference for horizontal centrifugation primarily arises from a limited understanding of how centrifuge mechanics truly work. Many clinicians are unaware that RPM alone does not determine PRF quality. Instead, the combination of rotor angle, tube position, and the resulting g-forces plays the most significant role in the biological outcome. When rotor geometry is not taken into account, practitioners may unintentionally expose tubes to g-forces far higher or lower than intended, which can negatively impact fibrin structure, platelet distribution, and overall PRF consistency.

To help clarify these misconceptions, this independent study provides a detailed comparison of the forces generated in various centrifuge designs and explains how these differences directly influence PRF quality. The research highlights why Dr. Choukroun’s fixed-angle spin protocols, which were scientifically developed, validated, and widely replicated, consistently yield superior fibrin architecture, higher platelet retention, and more predictable clinical results than those produced by horizontal or swing-out rotor systems. Clinicians seeking evidence-based guidance will gain valuable insight into how proper rotor selection and true g-force calculations contribute to producing the highest-quality PRF.

A Standardized g-Force Allows the Preparation of Similar Platelet-Rich Fibrin Qualities Regardless of Rotor Angle

What's Included in the Kits?

Blood Collection Supplies

• PRF-APRF+ - Box of 100 Glass A-PRF™ Membrane Tubes, High-Vacuum, No Additives
• PRF-SPRF - Box of 24 Plastic S-PRF™ Sticky Bone Tubes, High-Vacuum, No Additives
• PRF-PRVT2 - Box of 35 Butterfly 21-Gauge Blood Collection Sets

PRF Instrumentation and Equipment 

Starter Kit (One of Each) 

Professional Kit (Two of Each)

• PRF-POMPAC – Dr. Choukroun’s PomPac™ Cooling Tube Rack, 6 Hole
• PRF-POMCOL - Choukroun’s PomPac™ Blood Draw Cooling Pack
• PRF-TOURNIQUET - Dr. Choukroun’s Reusable Tourniquet
• PRF- EGTRAY - Dr. Choukroun’s EG Tray Sterilization Cassette – Does not fit PRFBOX
• PRF-PRFBOX - Dr. Choukroun’s Premium MK2 A-PRF™ Membrane and Plug Press Box
• PRF-MEMTRAY – Dr. Choukroun’s A-PRF™ Press / Sticky Bone Formation Reservoir
• PRF-ST-UP2 – Dr. Choukroun’s Periosteal Release Soft Brushing Instrument
• PRF-BIGCOMPACT- Dr. Choukroun’s Large Graft Packer 6/8mm, Serrated
• PRF-SMALLCOMPACT - Dr. Choukroun’s Small Graft Packer 3/5mm, Serrated
• NX-DSPOONTG – Dr. Choukroun’s Double-Ended Spoon, Gold Ion Edge® Surface
• PRF-PAD - Dr. Choukroun’s A-PRF™ Transportation Pad
• PRF-FORCEPS - Dr. Choukroun’s A-PRF™ PRF Forceps, 1x2 Teeth
• PRF-CSX – Dr. Choukroun’s PRF Scissors, Curved, Serrated
• PRF-TUBEHOLDER - Dr. Choukroun’s PRF Tube Holder, 12 Hole
• PRF-TRAY - Dr. Choukroun’s PRF Stainless Steel Tray
• PRF-BOWL - Dr. Choukroun’s Stainless Steel PRF Bowl

Optional Additions

Duo Quattro™ Advanced PRF System Applications

• Guided Bone Regeneration [31]
• Guided Tissue Regeneration [20]
• Gingival Recession [27]
• Sinus Augmentation [25]
• Socket Preservation [21]
• Facial Esthetics [32]
• Orthopedics [32]
• Dermal Regeneration [32]

Features & Specifications

• 2-year Warranty
• Brushless Centrifuge Motor
• Self-Calibrating Rotor
• 6 Pre-Programmed Settings using Dr. Choukroun's Protocols
• High-Vacuum Tube Design
• PomPac™ and PomCol™ Tube and Blood Draw Cooling System
• 110/220v Operation
• 12 Tube Capacity
• 41.3° Rotor Angulation
• FDA Cleared (3007006186)
• Centrifuge Dimensions: (12x11x8.5in) L x W x H

Why the Brand of PRF Tube Matters

Current research makes one point exceptionally clear: the brand and quality of the PRF tube influence PRF outcomes more than the centrifuge’s rotor angle, device type, or orientation. One of the most widely referenced independent studies—conducted by Al-Waawi, Dohle, Kretchmer, Sader, Rutkowski, and Ghannati—demonstrated that tube design, internal surface characteristics, and collection vacuum play a far greater role in PRF quality than whether a centrifuge uses a fixed-angle or horizontal rotor. Those results can be reviewed here.

This means a clinician can purchase the correct centrifuge, follow the correct spin protocol, and still produce inferior PRF simply by using the wrong tubes. Tube quality is not a minor variable; it is the most important determining factor when producing PRF.

The same study examining rotor angulation and tube design also dispels a common misconception: horizontal centrifugation does not improve plasma potency, volume, or biological performance. Many claims made by competitors are unsupported by any independent, third-party literature and often rely on internal studies conducted without outside evaluation. By contrast, Process for PRF™ prioritizes scientific transparency and relies extensively on unbiased, peer-reviewed research to validate every product they introduce.

Because tube quality directly drives PRF outcomes, the choice of tube brand must be based on biological evidence—not marketing claims or visual similarity.

A-PRF™ and S-PRF™ Tubes Stand Apart Because They Are:

• Silica-Free – Manufactured without additives or pro-coagulants that may interfere with the natural healing cascade.
• High-Vacuum Glass – Ensures rapid and complete blood draw, preserving fibrinogen and reliably increasing plasma yield.
• Micro-Textured Interior – Optimized to enhance growth factor release, concentrate leukocytes, support mesenchymal stem cell activity, and prevent premature coagulation. This design ensures that S-PRF™ remains potent and stays liquid longer than imitation or off-brand tubes.
• Biologically Validated – Supported by decades of peer-reviewed science, independent testing, and clinically reproducible outcomes.

Why Off-Brand Tubes Fail

Generic red-top or blue-and-white top tubes vary dramatically in:

• Vacuum pressure
• Internal surface texture
• Coating composition
• Activation properties
• Manufacturing consistency

These inconsistencies lead to unpredictable clot size, weak fibrin scaffolds, lower platelet concentration, and unstable PRF membrane formation. Even when used in a high-quality centrifuge, these tubes produce substandard biological performance and unreliable clinical results.

If your goal is to produce high-quality, biologically potent PRF, the tubes must be clinically validated—not merely “compatible.” The tube is the foundation of every PRF protocol, and choosing the correct brand is the single most important decision a clinician can make.

 

Comprehensive Support and Education Included

Every purchase of a DuoQuattro™ PRF System through HampMED  or any authorized Process for PRF™ distributor includes exclusive access to the world’s leading PRF education network:

• Free 8-hour comprehensive PRF course taught by the inventor of PRF, Dr. Joseph Choukroun - Call (918) 393-2664 after purchase to access the course at no charge.
• A continually updated online portal consisting of:
• Clinical protocols
• Case guides
• Video demonstrations
• Scientific literature
• Direct clinical and technical support via phone, email, or video call.
• Access to online or hands-on courses and mentorship opportunities.

This ensures you are not simply purchasing tubes; you are joining a global community of clinicians dedicated to evidence-based regenerative dentistry.

 

Notable Educators of Dr. Choukroun's System

Blood Collection Using the PomPac™ and PomCol™ System

To maximize platelet volume and potency, it is essential to utilize the PomPac™ tube cooling system and the PomCol™ blood draw cooling system. Store your tubes, blood draws, PomPac™, and PomCol™ in the refrigerator until the time of surgery. Right before surgery, take your tubes, blood draws, PomPac™, and PomCol™ out of the refrigerator, and place your tubes into the PomPac™. You want your tubes, blood draw, PomPac™, and PomCol™ around 40°F (3°C) to maximize the effectiveness of your PRF.

1. Remove 4-6 tubes of any kind from their packages and place them in the PomPac™. A maximum of 6 tubes can be drawn without needing to flush the line with saline. This also ensures that you complete your blood draw quickly, reducing fibrinogen death. It is critical to complete your blood draw as quickly as possible. 

2. Begin a blood draw with a 21-gauge blood collection butterfly needle. Once the needle is inserted, wrap the patient’s arm with the PomCol™.

3. While keeping the resting tubes in the PomPac™, remove one tube and begin to fill it. Once the tube is filled, place it back in the PomPac™. Make sure that when you draw blood into your tubes, the blood is filled to the same level in each tube. We recommend drawing blood to the top of the label on the tube. This ensures that your centrifuge rotor does not experience harmful vibrations, which reduces the volume of PRF that you produce.

4. Once all the tubes are filled, they can then be placed into the DuoQuattro™ Centrifuge. Tubes must be placed parallel to each other to eliminate harmful vibrations. Always put the same color tubes opposing each other when placing them into the DuoQuattro Centrifuge. You want to spin at least two green tubes and two red tubes at a time for dental applications. For aesthetics and injections, no more than six tubes should be drawn. If you have a red tube in the 12 o'clock position, put another red tube in the 6 o'clock position. If you have a green tube in the 3 o'clock position, place another green tube in the 9 o'clock position.

5. Red A-PRF™ tubes should be left to rest with the red tops removed for at least 5 minutes in the tube holder before removing the A-PRF™ membrane from the tube. Removing the membrane before 5 minutes will significantly reduce the mass and size of your A-PRF™ clots. Green S-PRF™ tubes can be used right away, but if you wish to mix the exudate produced by pressing A-PRF™ membranes with your S-PRF™ at the same time, you can place the green tubes back into the PomPac™ to prevent coagulation.

Why Proper Blood Draw Techniques and Tube Cooling Matter

 

Case Studies

References

[20] Fujiooka-Kobayashi M, Miron RJ, Hernandez M, Kandalam U, Zhang y, Choukroun J. Optimized Platelet Rich Fibrin With the Lowe Speed Concept: Growth Factor Release, Biocompatibility and Cellular Response. J Periodontal 2017;88(1):112-121. Epub 2016 Sep 2.

[21] Hoaglin DR, Lines GK, Prevention of localized osteitis in mandibular third-molar sites using platelet-rich fibrin. International journal of dentistry. 2013;2013:875380

[25] Zhang Y, Tangl S , Huber CD, Lin Y, Qiu L, Rausch-Fan X, Effects of Choukroun’s platelet-rich fibrin on bone regeneration in combination with deprotonized bovine bone mineral in maxillary sinus augmentation: a histological and histomorphometric study. Journal of cranio-maxillo facial surgery: official publication of the European Association for Craino-Maxillo-Facial Surgery. 2012;40(4):321-8

[27] Agarwal SK, Jhingran R, Baines VK, Srivastava R, Maden R, Rizvi I. Patient-centered evaluation of microsurgical management of gingival recession using coronally advanced flap with platelet-rich fibrin or amnion membrane: a comparative analysis. European journal of dentistry. 2016;10(1):121-33

[31] Miron RJ, Fujioka-Kobayashi M, Bishara M, Zhang Y, Hernandez M, Choukroun J. Platelet-Rich Fibrin and Soft Tissue Wound Healing: A systematic Review. Tissue engineering Part B, Reviews.2016.

[32] Kim DH, Je YJ, Kim CD, Lee YH, Seo YJ, Lee JH, et al. Can Platelet-rich fibrin be used for skin rejuvenation? Evaluation of effects of platelet-rich fibrin on human dermal fibroblasts. Annals of dermatology. 20122;23(4):424-31